gblocks gene fragments gblock-1  (Benchling Inc)

Journal: Frontiers in Immunology

Article Title: Preservation of functionality, immunophenotype, and recovery of HIV RNA from PBMCs cryopreserved for more than 20 years

doi: 10.3389/fimmu.2024.1382711

Figure Lengend Snippet: Representative flow plots from original four-colour flow cytometry performed on whole blood samples during 1995–1997. (A) Immunophenotype gating for CD45RA+ and CD45RO+ CD4 T cells. (B) Expansion of highly activated CD38+HLA-DR+ CD8 T cells during early untreated HIV-1 infection compared with an uninfected control.

Article Snippet: Quantification of HIV-1 copy number was determined with a standard curve generated with a HIV-1 gBlock gene fragment (Integrated DNA Technology, Coralville, Iowa, USA): 0.64-2000 HIV-1 copies/µl.

Techniques: Flow Cytometry, Infection, Control

Journal: Scientific Reports

Article Title: Rapid Detection of Mobilized Colistin Resistance using a Nucleic Acid Based Lab-on-a-Chip Diagnostic System

doi: 10.1038/s41598-020-64612-1

Figure Lengend Snippet: Sequence alignment, primers location and mcr −9 LAMP assay performance. ( A ) Sequence alignment for mcr −1 to mcr −9 showing priming region and the location of the FauI restriction site. Compared to the reference sequence, mcr −9, mismatches in the alignment are displayed as AGTC. Matched nucleotides are represented as dots and gaps are represented as dashes. ( B ) Standard curve and real-time amplification profiles obtained with the mcr −9 LAMP assay and synthetic DNA at concentrations ranging from 10 2 to 10 7 copies per reaction (including non-template control). Experiments were carried-out in a real-time qPCR platform in triplicates. ( C ) Gel electrophoresis confirming specificity of mcr −9 LAMP assay. Line 1 shows a 100 bp DNA ladder. Lines 2 to 10 show the amplification product obtained from synthetic DNA carrying mcr −1 to mcr −9 sequences against the mcr −9 LAMP assay. Line 11 shows the specific mcr −9 LAMP product after digestion with FauI .

Article Snippet: Double-stranded synthetic DNA (gBlock Gene fragments) containing mcr −1 to mcr −9 gene sequences (ranging from 1,617 to 1,898 bp) were purchased from Life Technologies (ThermoFisher Scientific) and resuspended in Tris-EDTA buffer to 10 ng/μL stock solutions (stored at −20 °C until further use).

Techniques: Sequencing, Lamp Assay, Amplification, Control, Nucleic Acid Electrophoresis

Journal: Cell chemical biology

Article Title: A Split-Abl Kinase for Direct Activation in Cells

doi: 10.1016/j.chembiol.2017.08.007

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-FLAG M2 and Anti FLAG HRP Conjugate Sigma #F1804 & #A8592 Anti Src Thermo-Scientific MA5-15120 Anti-Phospho-Src Y416 Cell Signaling Technology #2113 Anti-Phospho-Abl Y412 Cell Signaling Technology #2865 Phospho-Y-100 & Phospho-Y-1000 Cell Signaling Technology #9411 & #8954 Anti-GAPDH Cell Signaling Technology #14C10 Anti-Myc Tag (mouse & rabbit) Cell Signaling Technology #2276 & #71D10 Anti-Phospho-AktT308 & S473 Cell Signaling Technology #13038 & #4060 Anti-p70 S6 Kinase & phopspho-p70 S6K (T389) Cell Signaling Technology #9202 & #9234 Goat anti-Mouse-HRP Thermo Scientific #31430 Goat anti-Rabbit-HRP Thermo Scientific #31460 Alexa Fluor 488 anti-mouse # {"type":"entrez-nucleotide","attrs":{"text":"A11029","term_id":"492395","term_text":"A11029"}} A11029 Life Technologies # {"type":"entrez-nucleotide","attrs":{"text":"A11029","term_id":"492395","term_text":"A11029"}} A11029 Alexa Fluor 647 anti-rabbit Life Technologies # {"type":"entrez-nucleotide","attrs":{"text":"A21245","term_id":"641367","term_text":"A21245"}} A21245 Bacterial and Virus Strains DH5 alpha, subcloning efficiency Life Technologies #18265017 Biological Samples Chemicals, Peptides, and Recombinant Proteins Rapamycin Cayman Chemicals #53123-88-9 Imatnib Selleckchem #S2475 NeuCode labeling reagents: L-Lysine:2HCl (3,3,4,4,5,5,6,6-D8, 98%)) and L-Lysine:2HCl (13C6, 99%, 15N2, 99%) Cambridge Isotopes #DLM-2641-0 & #CNLM-291-H-0.25 Critical Commercial Assays Deposited Data Experimental Models: Cell Lines Human: HEK293 Cells UCSF Cell Core Human: HeLa Cells UCSF Cell Core Experimental Models: Organisms/Strains Oligonucleotides Primers & gBlocks Gene Fragments IDT Recombinant DNA pQCXI P/H Clontech 631515 pcDNA3.1 Invitrogen V790-20 Software and Algorithms Prism 6 Graphpad Excel Microscoft Office 2016 for Mac Proteome Discoverer Thermo Fisher NeuQuant, Opensource Software Baughman et al. 2016 PhosphoRS/COMPASS Taus et al. 2011 Other Lipofectamine 2000 Life Technologies 11668019 Open in a separate window Key Resources Table A split protein variant of cAbl kinase for rapid cellular activation Neucode-based phosphoproteomics characterizes selective activation Known and novel cAbl kinase downstream targets are identified and discussed

Techniques: Subcloning, Recombinant, Labeling, Software